GR Recombinant Egg Basic Information:
GR recombinant egg (Glucocorticoid receptor) is a hormone receptor protein that plays a key physiological function in the human body. It is a structurally specific protein that includes a DNA-binding domain, a hormone-binding domain, and a transcriptional activation domain. GR recombinant eggs mainly bind to DNA in the nucleus and participate in the regulation of gene expression, thereby affecting physiological processes such as cell growth, proliferation and differentiation.
Catalog No. PA1000-8506
Introduction to Structural Functions:
The structure of the GR recombinant egg is very complex, containing a DNA-binding domain, a hormone-binding domain, and a transcriptional activation domain. Among them, the DNA binding domain mainly interacts with DNA non-specifically, and the target gene is identified through DNA binding. The hormone-binding domain is the part of the GR recombinant egg that binds to hormones, and in the absence of hormones, it binds to nuclear hormone receptor-associated protein (HSP) to form a complex. When there is hormone binding, the complex changes, and the hormone-binding domain is exposed, which activates the activity of GR. The transcriptional activation domain mainly regulates the interaction between GR and other transcription factors and regulates the expression of genes.
Biological Activity Verification Methods:
Bioactivity validation typically assesses the activity of GR recombinant eggs through changes in gene expression at the transcriptional level. Commonly used validation methods include transcription-level luciferase reporter assays, real-time PCR, and more. Luciferase reporter assay is a commonly used method to indirectly assess the activity of GR recombinant eggs by linking the luciferase gene to the gene expression structure of interest, and using the expression amount of the luciferase gene to reflect the expression level of the gene.
In general, the function of the molecule in the organism is very important, and it can affect many important physiological processes in the cell. Through appropriate biological activity verification methods, we can better understand the activity and structure of GR recombinant eggs, so as to provide guidance and reference for related research, which is helpful to further study the mechanism of action of GR recombinant eggs in cells, and provide new ideas and directions for related diseases.