A concentrator is an experimental instrument used to concentrate a liquid sample, which can remove the solvent in the solution through evaporation and condensation, so as to obtain a concentrated sample.
Here are the steps to use the concentrator:
1. Preparation: Before use, first make sure that the required equipment, reagents and consumables are complete. Check whether all parts of the instrument are in good condition, such as condenser, sampling bottle, vacuum pump, etc.
2. Set parameters: set the corresponding parameters of the instrument according to the experimental requirements. Including evaporation temperature, condenser temperature, rotation speed, etc. According to the characteristics of the reagent and the concentration of the sample, the parameters are set reasonably to achieve the best concentration effect.
3. Preheating: Before concentrating the sample, the concentrator needs to be preheated. Turn on the power of the instrument and adjust the temperature setting so that the device is at operating temperature. The warm-up time is usually 10-15 minutes.
4. Sample preparation: Add the sample to be concentrated to the concentration flask. Depending on the size of the sample volume, select the appropriate concentrator capacity. Make sure the sample does not exceed the mouth of the bottle and try to avoid air bubbles.
5. Start the concentrator: Place the concentrator in the heating area of the concentrator, and then turn off the sealing device of the instrument. Turn on the vacuum pump and start pumping the solvent from the sample. The adjustment knob allows the instrument to achieve the appropriate rotational speed to promote solvent evaporation and condensation.
6. Monitor the concentration process: In the process of sample concentration, the working status of the instrument should be monitored at any time. Pay attention to the temperature and pressure changes of the sample in the concentrator and observe if there is any abnormality.
7. Stop concentration: When the solvent in the sample is concentrated to a predetermined concentration, the work of the instrument can be stopped. First turn off the vacuum pump, then turn off the heating unit. After waiting for the instrument to cool down, open the sealing device of the instrument and remove the concentrated sample.
8. Cleaning and maintenance: After using the concentrator, clean all parts of the instrument in time to avoid the accumulation of residues. Pay attention to the maintenance and maintenance according to the requirements of the instrument to prolong the service life of the instrument.
The above are the steps to use the concentrator. During operation, it is necessary to strictly abide by the safety regulations of the laboratory, pay attention to personal protection, and ensure that the use process is safe and reliable. At the same time, according to the requirements of the experiment and the characteristics of the sample, the appropriate concentration method and parameters are selected to obtain the ideal concentration effect.