In experiments, experimenters often have to repeat an experimental project, and the repeated steps in some experiments are gradually developed by researchers as "substitutes" with the progress of science and technology. These "substitutes" do repetitive steps or delicate work. Here is a product that can not only save the time cost of experimenters, but also protect experimental animals from the pain of repeated injections and injections. This product is the Alzet microosmotic capsule pump introduced by Alzet in 1977 for the extensive experimental market.
This product has been facing the market for nearly 50 years, with the development of the times, Alzet company has also undergone several reforms to the product, the manual has also changed with the change of the product, in some details have also changed, let us enter the world of the Alzet osmotic pump manual to see, the operation of the product and the key points to pay attention to.
Alzet Osmotic Pump Instruction SheetIn the experimental operation, the Alzet osmotic pump has two major steps, one of which is the filling capsule pump: when filling the capsule pump, we first disinfect the hands and the experimental environment, and then weigh the weight of the capsule pump and the pump head for subsequent experimental calculations. Subsequently, use a 1ml syringe for drug filling, during injection filling, pay attention to the connection between the pump and the syringe do not produce bubbles, unplug the flow regulator, stand the osmotic pressure pump upright, and insert the perfusion tube from the small opening at the top of the osmotic pressure pump until it can no longer be inserted; At this time, the tip of the perfusion tube is close to the bottom of the capsule, keep the osmotic pump upright, slowly push the piston of the syringe until the drug solution reaches the small opening at the top of the pump, stop perfusion when the drug solution reaches the small opening at the top of the capsule, and carefully remove the perfusion tube; Wipe off the spilled drug solution and insert the flow regulator until the white cap reaches the top of the osmotic pump. Finally, weigh the overall weight of the capsule pump and calculate. The second is the site of the alzet capsule pump implantation surgery and the requirements for experimental mice, this step is recommended not to rely on the product manual, the text description may not be you can understand, it is recommended that for the implantation surgery, it should be operated according to the experiment, the operation is easier to understand than the text.
Alzet Osmotic Pump Instruction SheetThe key points of attention, the precautions of the Alzet osmotic pump must attract our attention, the operation details are the success or failure of the experimental results, the slightest miss, the difference of thousands of miles. In the whole process of the experiment, we must pay attention to the aseptic operation of the experiment, which is the top priority of the experiment. In addition, we should also pay attention to the generation of bubbles, the disposable use of the product, the use temperature, volume calculation, etc. In the experiment, we must read the product manual carefully, and some words in the manual play a great role in the experiment.