We brought it up with our fans"How to Achieve Single Particle Capture in Solution".specially entered the University of Science and Technology of China to conduct an exclusive interview with Professor Chu Kaiqin.
The professor explained that nanoscale particles are usually swimming in solution, and that capturing and characterizing single particle parameters is critical in some detection needs with high single-particle heterogeneity. Developed by the professor's team[Nanoparticle multi-parameter characterization and analysis system].One of the characteristics is the realization of nanoscale single particulate matterAutomatic capture and release, and further achieved the rapid characterization of single particles and multiple parameters of nanoparticles with the help of Raman signal analysis.
How? According to Professor Chu, they designed it in the system"Multimodal Raman Optical Tweezers System"., a spatially filtered high-quality Gaussian beam that is focused from the objective lens onto the particles and captures them. The back-Raman scattering signal from the ** obtained particles is separated into the confocal collection module through a dichroic mirror, and the optical fiber is used as the confocal diaphragm to filter out the interference noise other than the detection focal spot, and the optical fiber is connected to the Raman grating spectrometer and the Raman spectrum is obtained with a high-sensitivity back-illuminated CCD camera. The opening and closing of the shutter in the optical tweezer system realizes the capture and release of the sample.
To put it in layman's terms,The Gaussian beam acts like a tweezer that grips a single particle in a swimming state, and when the grating is closed, it is released.
In addition, they created a sample stage and objective adjustment frame to carry and adjust the levelness of the sample to the objective. It is completed by connecting and integrating the optical tweezers system, the confocal Raman signal collection module, and the high-speed darkfield imaging system for the capture of individual particles and rapid multi-modal data acquisition.
The following is a schematic diagram of the structure:
This is an introductory article on the three Raman analysis systems in the previous issue:
Raman signal analysis technology supports "label-free" to lead a new paradigm of biochemical detectionHere's what we're rolling out:
Provide sample testing services, you can get scientific analysis result data and analysis maps without spending high cost on the purchase of instruments and equipment.
Provide mature system construction services, we will build an optical system with the above detection functions in your laboratory according to your needs and provide supporting algorithm software.