MUC3 is an important mucosal adhesion protein, which is an important component of mucosal surface, mainly distributed in the digestive tract and respiratory tract. MUC3 is a glycoprotein with a polysaccharide structure, and its main function is to provide a protective mucus barrier and maintain moisturizing and defensive effects on the mucosal surface. The abnormal expression of MUC3 is closely related to a variety of diseases, such as inflammatory bowel diseases and bowel cancer, so the study of MUC3 has important biological and clinical significance.
Catalog No. PA1000-8843
Recombinant eggs of MUC3 are prepared from eukaryotic cells by engineering techniques and have similar structures and functions to native MUC3. Verification of biological activity is one of the important methods for functional evaluation of recombinant eggs. Commonly used validation methods include bioactivity assays and structure-function analysis.
Validation methods for biological activity mainly include adhesion assays, adhesion antigen binding assays, and immunoactivity assays. Adhesion assays evaluate the adhesion performance of MUC3 protein by contacting a specific protein or cell to observe its ability to adhere to the surface. The adhesion antigen binding test evaluates the adhesion performance of the muc3 protein by binding it to a specific antigen and detecting its ability to bind. The immunoactivity assay is to evaluate the immunomodulatory effect of MUC3 protein by detecting its effect on the immune system.
The detailed description of structure and function mainly includes the composition, structure and biological function of MUC3 protein. MUC3 protein is composed of two parts, glycan and protein, and the glycan part mainly includes various glycosylation modifications, among which N-glycan and O-glycan are the key structures of MUC3 recombinant eggs. The protein part mainly includes the protein domain of MUC3, including the core domain, cross-linking domain and CTERM domain. Together, these domains are involved in the adhesion properties and immunomodulatory effects of MUC3.
Overall, MUC3 recombinant eggs are an important bioactive protein, and verification of their biological activity can be performed by a variety of methods, including adhesion assays, adhesion antigen binding assays, and immunoactivity assays. A detailed introduction to the structure and function can help us better understand the biological properties and functions of the MUC3 protein. The study of MUC3 is helpful to deeply understand its mechanism of action in mucosal protection and immune regulation, and provides an important theoretical basis for the prevention and treatment of related diseases.