Introduction
Gene synthesis refers to the technology of artificially synthesizing double-stranded DNA molecules in vitro, which is different from oligonucleotide synthesis: oligonucleotides are single-stranded and can synthesize long fragments of only about 100nt, while gene synthesis is the synthesis of double-stranded DNA molecules, which can be synthesized in the length range of 50bp-12kb. Gene synthesis is the technology of synthesizing genes by artificial methods, which is one of the means of gene acquisition, compared with obtaining genes from existing organisms, gene synthesis does not require templates, so it is not limited by genes.
Gene synthesis is the foundation of molecular biology research, and no matter how far-reaching our research is, it starts with basic gene synthesis. Through gene synthesis, genes that do not exist in nature can be obtained, opening up a completely new direction for the biomedical field. With the continuous innovation of technology, gene synthesis plays an increasingly important role in the field of life sciences, and its role in new energy, new materials, artificial life, nucleic acid vaccines, biomedicine and other fields has been initially reflected.
Gene synthesis is the main content of current synthetic biology, through gene synthesis, genes that do not exist in nature can be obtained, opening up a new direction for human modified organisms, and gene synthesis will play a huge role in the field of life sciences in the foreseeable future. There are two ways of gene synthesis, one is to order from a gene synthesis company, and the other is to do local gene synthesis. Since there is no unified method for gene synthesis technology, and the skills and experience of gene synthesis have a decisive impact on the synthesis process, most of the gene synthesis is done in the hands of professionals, which also determines the main channel of route 1.
With the advantages of mature and perfect whole gene synthesis technology and advanced production management mode, we are committed to providing customers with fast and accurate gene synthesis services. The business involved is whole gene synthesis, subcloning, point mutagenesis, plasmid preparation and combination business (such as gene synthesis + subcloning, subcloning + plasmid preparation, etc.), with a wide range of customer groups.
Contents
1 Genetic analysis.
Use sequencing platforms to capture information and analyze cellular data collected through genomics, transcriptomics, metabolomics, and fluxomics.
2 Clone builds.
The gene of interest is constructed onto a cloning vector.
3 Genetic testing of interest.
Gene sequencing testing verifies the accuracy of genes.
4 Large-scale cloning of the gene of interest.
5 Deliverables (negotiable).
COA file - plasmid structure diagram.
Sequencing maps. Approximately 5 ug of high-purity plasmid dissolved in ebbuffer.
One aliquot of glycerol bacteria containing recombinant plasmids.
Fields of application
Gene synthesis technology can be applied to:
Clone a human mouse antibody or a recombinant antibody.
Synthesis of different mutant strains, SNPS, or other mutant strains.
Design synthetic DNA vaccines.
Synthesis of cDNA for microchips in large quantities
Advantages:
Gene Synthesis Advantages:
Optimize genes for improved performance and protein expression.
Cloning outsourcing saves time and manpower, allowing you to focus on discovery.
The customization feature allows you to order and get the exact sequence of interest.
Accuracy guaranteed—your clone will be an exact match to the sequence ordered (without the risk of extraneous mutations).
Ability to construct complex challenging structures and custom vectors.
Multiple variants can be generated, making it suitable for comparative studies of wild-type and mutant types.
The team has a wealth of experience and can provide a variety of service advantages:
Normally difficult gene sequences of any sequence and length can be synthesized according to customer requirements, including high AT;High GC;Hairpin construction;Repetitive sequences;Continuous single-base repeats as well as difficult sequences of toxic genes that replicate unstable ones.
The optimization of gene design schemes, codons, and enzyme cleavage sites greatly improves the level of gene expression.
The synthesis cycle is short, saving customers valuable time.
To ensure high accuracy, all synthesized genes are analyzed by sequencing and a sequencing report is provided.