NHS SS biotin, activated lipid disulfide biotin crosslinker

Mondo Health Updated on 2024-01-30

Crosslinker refers to a class of reagents that have the same or different active groups at both ends of a molecule, and they can covalently combine with other amine groups, sulfhydryl groups, hydroxyl groups, etc. on other molecules to produce cross-linking reagents, cross-linking agents can be divided into three types: homobifunctional reagents, heterobifunctional reagents and photoactivating reagents. Cross-linking reaction has become an important means to study the structure, function and interaction of protein subunits, which can provide a lot of important information about protein conformation, subunit composition, structure and function and interaction, and is also an important raw material for the synthesis of functional polymer materials.

Protein cross-linking, modification, and labeling are common techniques for studying protein structures and interactions. Crosslinking refers to the process of chemically combining two or more molecules through covalent bonds. Modification refers to the joining or cleavage of chemical groups in order to alter the solubility or other properties of the original molecule.

Product name: NHS-SS-Biotin

Product Name:Biotin Disulfide N-Hydroxysuccinimide ester

Chinese name: activated lipid disulfide bond linked biotin.

cas #nhs-ss-biotin, succinimidyl-2-(biotinamido)-ethyl-1,3'-dithiopropionate, is an amine-reactive biotinylation reagent that contains a reducible disulfide. nhs-ss-biotin is cell membrane per-meable and has a long chain separating the biotin group from the amino-reactive nhs-ester (n-hydroxysuccinimide ester). this product is often used to purify an antibody or other protein from a biotin specific affinity resin like immobilized strept**idin without exposing your protein of interest to denaturing conditions.

biotinylation reagent incorporating a spacer with a cle**able disulfide linkage. typically coupled to primary amine in the ph range 6.5-8.5. cle**age of disulfide requires reducing agent allowing mild recovery of biotinylated compound.

Appearance: White powder.

molecular weight: 504.7

MF: C22H33N5O7S3

Solubility: DMSO

cas #spacer length: 24.3 å

alternative name: biotin disulfide n-hydroxysuccinimide ester

Purity: 98%+

Save: -20

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Editor: Qi Yue Hao, December 20, 2023.

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