Heparin fluorescently labeledcyanine3-heparin
Cyanine3-Heparin is a fluorescent dye-labeled heparin with high sensitivity and stability, which can be widely used in biomedical research fields.
1. Synthesis method of cyanine3-heparin.
Cyanine3-Heparin is synthesized by conjugation of Cyanine3 fluorescent dye to a heparin molecule. The reaction is at pH 74 phosphate buffers are performed to facilitate the reaction between the fluorescent dye and the heparin molecule. After sufficient reaction, the target product cyanine3-heparin can be obtained.
2. Labeling principle of cyanine3-heparin.
The labeling principle of Cyanine3-Heparin is based on the fluorescence resonance energy transfer (FRET) effect. When Cyanine3-Heparin is excited by excitation light, its fluorochrome molecule absorbs the light energy and converts to an excited state. In the excited state, the fluorochrome molecule will transfer energy to adjacent biomolecules through the FRET effect, thus enabling the labeling of biomolecules.
3. Application fields of cyanine3-heparin.
Cyanine3-Heparin is widely used in biomedical research as a fluorescent label with high sensitivity and stability. For example, it can be used to label cell surface proteins, detect biomolecular interactions, fluorescence in situ hybridization (FISH), fluorescence energy resonance transfer (FRET), etc. In addition, Cyanine3-Heparin can also be used to fabricate fluorescent heparin sensors for the detection of biomolecular and cellular activity.
4. Advantages and disadvantages of cyanine3-heparin.
Cyanine3-Heparin has the following advantages: high sensitivity, high stability, low toxicity and good photostability. However, it also has some drawbacks: the synthesis process is relatively complex and costly; The conjugation process may cause loss of activity of antigens and antibodies; Fluorescent dyes may be toxic to cells.