When I first entered the laboratory, there would be a big question mark in the cerebellum of some little whites, and I didn't know or understand some experimental materials and equipment. At this time, there will be senior brothers, senior sisters, and teachers who will popularize science for Xiaobai, but there are some that Xiaobai needs to search for by himself. If it is in a cell laboratory, there will be questions about cell culture, such as: what are the cells, precautions for cell culture, will we use fetal bovine serum, etcWhat is fetal bovine serum used for?
Here is a simple compilation of some small knowledge about fetal bovine serum for the whites, and the whites should pay attention to check it!
The main component of fetal bovine serum.
Various proteins: albumin, globulin, fibronectin, transferrin, etc.;
Factors: various growth factors, etc.;
Various hormones: insulin, somatotropin, hydrocortisone, etc.;
Other trace elements, vitamins, ions, etc.;
Unclear composition: Since fetal bovine serum is a natural biological product, there are still some unclear biological components in FBS.
Advantages of fetal bovine serum.
Compared with other types of serum, fetal bovine serum is of the highest quality, because fetal cattle have not been exposed to the outside world, the serum contains the least harmful components such as antibodies to cells, and embryonic animal serum contains more growth factors. In addition, embryonic serum contains lower concentrations of complement than neonatal and adult serum. Complement has the ability to lyse cells*** and also interfere with immune detection.
The **ground of fetal bovine serum**?
There are many brands and types of serum on the market, domestic, Uruguayan, American, etc. When buying fetal bovine serum, we should not only look at the quality of fetal bovine serum, but also observe the best place of fetal bovine serum. Some countries have cases of mad cow disease (BSE) and foot-and-mouth disease (FMD) contamination, and understanding the best places can avoid problems in subsequent experiments. During the purchase process, we can confirm that fetal bovine serum does not come from countries contaminated with mad cow disease (BSE) and foot-and-mouth disease (FMD) by asking dealers, customer service, sales, etc.
What is fetal bovine serum used for?
Fetal bovine serum is used in stem cell research, animal diagnosis, biotechnology research and production, vaccine production, etc. Fetal bovine serum is most commonly used in cell culture. The addition of animal serum to the medium primarily helps to provide nutrients and energy**, growth and adhesion factors. In addition, it can protect cells and maintain optimal cell growth form.
The above is some small knowledge about fetal bovine serum, do you still have any questions, welcome to discuss and communicate!