Fetal bovine serum, as a key biological reagent, plays an important role in life science research such as cell culture and drug screening. In order to ensure the accuracy and reliability of experimental results, it is particularly important to properly store and thaw fetal bovine serum. This article will detail the storage and thawing methods of fetal bovine serum to help scientists better use this reagent.
1. Storage of fetal bovine serum Hot Spot Engine Plan
1.Temperature control: Fetal bovine serum should be stored in a low temperature environment below -20 to ensure its stability and extend shelf life. During storage, temperature fluctuations should be avoided so as not to affect the quality of the serum.
2.Avoid repeated freeze-thaw cycles: Repeated freeze-thaw cycles can lead to denaturation and precipitation of proteins in serum, which can affect its performance. Therefore, when taking serum, the number of freeze-thaw times should be minimized to ensure that it is fully used in one go.
3.Sterilization: In order to ensure the sterility of fetal bovine serum, it can be filtered and sterilized once after purchase. The treated serum can be used directly in cell culture without the need for re-sterilization.
2. Defrosting of fetal bovine serum.
1.Preheating: Before thawing fetal bovine serum, the water bath should be preheated to 37. The serum is then removed from the refrigerator and quickly placed in a water bath to thaw. Be careful not to allow the serum to come into direct contact with the bottom of the water bath to avoid local overheating due to uneven heat.
2.Shake gently: During the thawing process, the serum bottle should be shaken gently to ensure that the serum is heated evenly and to avoid the formation of ice crystals. At the same time, it also helps to speed up the thawing.
3.Aliquot and preserve: Thawed serum should be dispensed immediately and stored in the refrigerator at 4 °C. The aliquoted serum can be stored for a long time, avoiding the impact of repeated freeze-thaw on serum quality.
3. Precautions.
1.Avoid contamination: When storing and thawing fetal bovine serum, aseptic practices should be strictly adhered to to avoid contamination. At the same time, the appearance and color of the serum should be checked before use, and if there is any abnormality, it should be stopped in time.
2.Use appropriate amount: According to the experimental needs, the fetal bovine serum should be used reasonably. Excessive use is not only a waste of resources, but can also negatively impact experimental results.
3.Record management: For stored and thawed fetal bovine serum, detailed record management should be done. Include information such as storage date, thawing date, and amount of use, so that you can keep track of the use of serum at any time.
In conclusion, proper storage and thawing of fetal bovine serum is of great significance to ensure the accuracy and reliability of experimental results. Researchers should strictly follow the above methods to ensure the quality and safety of serum. At the same time, paying attention to the quality control of serum can also help improve the success rate and reliability of experiments. Through reasonable storage and thawing management, fetal bovine serum will play a greater role in life science research and promote the continuous progress of scientific research.