Chinese name: DBCO-water-soluble sulfocyanine dye cy5
Product Name: dbco-cy5
Synonyms: sulfo-cy5 dbco
cas：1564286-24-3
Appearance: Dark blue solid.
MF: C53H59N4F6O2P
Excitation wavelength: 646
Emission value: 662
cf260：0.03
cf280：0.04
Solubility: Good solubility in DMF, DMSO, chlorinated organic solvents.
Quality control: NMR hydrogen spectrometry, high performance liquid chromatography-mass spectrometry (95%)
Storage conditions: 12 months after reception in the dark at -20 °C.
Uses: Only for scientific research, not for human body.
DBCO-Cy5 is a water-soluble cyanine dye, Cy5, which is a dye with excellent optical properties and is commonly used in fluorescent labeling and bioimaging. It is characterized by high fluorescence quantum yield, excellent water solubility, low toxicity and high stability.
The synthesis of DBCO-Cy5 requires multiple steps, including the synthesis of the DBCO moiety and the ligation of the Cy5 dye moiety. Among them, DBCO is a dibenzocyclooctyne compound with high reactivity and stability, and can carry out copper-free click reactions with a variety of biomolecules. Cy5 dye, on the other hand, is a cyanine dye with excellent optical properties and stability, which can achieve fluorescent labeling on various biomolecules.
By linking DBCO and Cy5, we can obtain a water-soluble dye with excellent optical properties. This dye can be used in a variety of fluorescent labeling and bioimaging experiments, such as cell imaging, protein labeling, nucleic acid sequencing, and more. In addition, DBCO-Cy5 can also be used in drug screening, immunoassays, biosensors, and other fields.