AMY2A is a human salivary amylase gene encoding the salivary amylase A protein. Salivary amylase A is a digestive enzyme that is secreted mainly in the mouth and plays a role in hydrolyzing starch into glucose. AMY2A recombinant protein is an artificially synthesized salivary amylase A protein obtained by recombinant technology, which has similar biological functions.
Catalog No. PA1000-7036
The biological function of salivary amylase A mainly includes participation in the digestion process of starch, hydrolyzing starch into smaller sugar molecules, making it easier for the body to absorb and utilize. In addition, salivary amylase A also has an antimicrobial effect, which can help clean the bacteria in the mouth and maintain oral health.
To verify the activity of the AMY2A recombinant protein, enzyme-linked immunosorbent assay (ELISA) assay can be used. By detecting the activity of the recombinant protein on a specific substrate, its hydrolytic capacity for starch can be determined. In addition, in vitro digestion tests can also be used to simulate the digestion environment in saliva and observe the degradation of starch by recombinant proteins.
An experiment was designed to observe the degradation of starch over time by mixing AM2A recombinant protein with a certain concentration of starch solution in an in vitro digestion test, adjusting pH and temperature to suitable conditions. By measuring the concentration of glucose produced, the digestibility of the recombinant protein can be assessed and its biological function can be verified by comparing it with the activity of natural salivary amylase.